Ablation of P.gingivalis and P.intermedia using Nd:Yag laser in an in-vitro tissue model
Sneha GokhaleBDS Post-graduate student , Ashvini PadhyeMDS, Professor , S SumanthMDS, Senior Lecturer
Dept of Periodontology and Implantology, M.A. Rangoonwala College of Dental Sciences and Research Centre
Correspondence Address:
Source of Support: None, Conflict of Interest: None
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Background and Objectives: The purpose of the study was to evaluate the efficacy of Nd:YAG laser in destruction of Porphyromonas gingivalis and Prevotella intermedia and to assess surface damage after ablation with laser in an in vitro tissue model.
Materials and Methods: Subgingival plaque samples were collected from patients with Generalized Chronic Periodontitis with a sterile curette. P.gingivalis and P.intermedia were isolated and cultured on blood agar plate which was the test group, while blood agar plate without colonies was the control group. Ablation with Nd:YAG laser was done on both the plates and the results were observed under the Surgical Microscope under 24X magnification.
Results: Distinct bottomless craters were seen after ablation of the test group with Nd:YAG laser due to lysis of the Colony forming units. No change was noted on the surface of the control group after ablation with Nd:YAG laser.
Conclusion: Nd:YAG laser can selectively destroy pigmented perio-pathogenic bacteria leaving the surrounding tissue intact.